E ISSN: 2583-049X

International Journal of Advanced Multidisciplinary Research and Studies

Volume 3, Issue 4, 2023

Detection of Panton-Valatin Lecucidin and Meca Genes in Staphylococcus Aureus Isolated from Al-Najaf Patients

Author(s): Fatima Karim Nasser, Dr. Ebtehal Edrees Ahmed Shubber


Methicillin.-resistant. Staphylococcus. Aureus poses a significant. Challenge in managing the infections it can cause. The Panton-Valentine leukocidin (PVL) is a significant factor contributing to the virulence of Staphylococcus aureus (SA) and has been associated with potentially life-threatening infections. The aim of this study was to investigate the frequency of methicillin resistant Staphylococcus aureus (MRSA) within Staphylococcus aureus (SA) isolates, together with the detection of the Panton-Valentine Leukocidin gene (PVL). The utilization of the disk diffusion method was employed as a means to detect the presence of methicillin resistance. Both cefoxitin and oxacillin disks were employed for this purpose. The molecular investigation employed particular primers designed to target the mecA gene and pvl gene using the polymerase chain reaction (PCR) method. The results indicated that among the 30 isolates of Staphylococcus aureus (SA) that were diagnosed, 25 were determined to be strains of methicillin-resistant Staphylococcus aureus (MRSA). In addition, the MRSA isolates demonstrated resistance to both cefoxitin and oxacillin. According to the findings of the molecular investigation, it was observed that 83.3% of the isolates obtained from (SA) exhibited the presence of the mec a gene. Regarding the pvl gene, it was observed that 26.6% of the tested isolates yielded positive results, resulting in the identification of a total of 8 positive isolates.

Keywords: PVL Gene, Meca Gene, S Aureus

Pages: 1203-1208

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