DNA Fingerprinting and Polymorphism of Five Ocimum basilium Cultivars Detected using ISSR Markers

Current investigation was accomplished at biology department/faculty of science/kufa university during 2021-2022 to asses geneticvariation among five Ocimum basilicum L. cultivars 1-Iranian (green) 2- Iranian (red) 3-Eygptian (green) 4-Local (green) 5-Turkish (red) with diverse geographical origin using ten ISSR markers. Results showed that ISSR markers were effective in generating polymorphism in basile germplasm reached to 100% by primers UBC812 and UBC852 and givingunique fingerprint to three cultivars by eight primers. Phyllogenetic tree and genetic distance for studied cultivars are not strongly related to cultivar origin or morphology.