E ISSN: 2583-049X

International Journal of Advanced Multidisciplinary Research and Studies

Volume 3, Issue 4, 2023

DNA Fingerprinting and Polymorphism of Five Ocimum basilium Cultivars Detected using ISSR Markers

Author(s): Zahraa Hammoodi Hussein AL-Shujairi, Attyaf Jameel Thamir


Current investigation was accomplished at biology department/faculty of science/kufa university during 2021-2022 to asses geneticvariation among five Ocimum basilicum L. cultivars 1-Iranian (green) 2- Iranian (red) 3-Eygptian (green) 4-Local (green) 5-Turkish (red) with diverse geographical origin using ten ISSR markers. Results showed that ISSR markers were effective in generating polymorphism in basile germplasm reached to 100% by primers UBC812 and UBC852 and givingunique fingerprint to three cultivars by eight primers. Phyllogenetic tree and genetic distance for studied cultivars are not strongly related to cultivar origin or morphology.

Keywords: Ocimum basilium, ISSR, Phyllogenetic Tree, Fingerprint, Genetic Distannce

Pages: 634-638

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