Biochemical Evaluation of Serum Lipids and Cardiac Enzymes: Techniques and Diagnostic Value

The biochemical evaluation of serum lipids and cardiac enzymes is central to understanding cardiovascular health and disease. Lipid profile estimation, including cholesterol, triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL), provides insight into atherogenic risk. At the same time, enzymatic assays for cardiac markers such as creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponins, and aspartate aminotransferase (AST) enable the diagnosis of myocardial injury. This article reviews the key methods used in lipid and enzyme estimation, presents their strengths and limitations, and highlights their diagnostic value in clinical practice.