Isolation Characterization of phytosterol (3(β) Lup -20(29)en-3yl Benzoate) from the Leaf of Gongronema Latifolium Benth

Plants secondary metabolites tend to be of immense health benefit to man, the extraction and characterization of these compounds are of profound importance. Isolation and characterization of 3(β) Lup -20(29) en-3yl benzonate from the leaf of Gongronema latifolium Benth was done with the aid of column chromatography, thin layer chromatography IR spectrophotometer, 1H and 13C NMR and mass spectrometer. IR Spectrum was obtained from a thermo Nicolet Nexus 470 FR-IR spectrometer. The IH and 13C NMR spectra were obtained on a Bruker Avance 400 FT NMR Spectrometer using tetra methyl silane as internal standard. Chemical shifts are expressed in ð values. LC - ESIMS spectra where determined in the positive ion mode on a PE. Biosystem APl 156 single quadruple instrument. HRESIMS (positive ion mode) spectra were recorded on a thermo Finniga MAT 95 x L. 3(β) Lup -20(29) en-3yl benzonate was identified having m/z 530. Lupeol and its derivatives are phytosterols which acts as therapeutic and chemo-preventive agents for the treatment of inflammation and cancer.