Factors Affecting the Success Rate in in vitro Grafting of Grapefruit Trees (Citrus spp.)

Tissue culture is an effective technique in propagating disease-free citrus plants and conserving valuable varieties. This study aims to evaluate the factors affecting the success rate of in vitro grafting of grapefruit trees (Citrus spp.).) in vitro. The results show that Calcium hypochlorite treated for 10 minutes is the most effective disinfectant (100% sample cleanliness). The Rough Lemon rootstock has the highest compatibility rate with the Citrus reticulata buds (88.9%) and is suitable for many other bud varieties. Regarding the culture medium, a BA concentration of 0.1 mg/L resulted in the highest survival rates for all three bud varieties, with Da Xanh achieving 66.67% and ???ng Lá Cam achieving 55.55%. The results provide a basis for the micrografting process applied in the propagation of disease-free grapefruit plants.