Antibiogram of Bacterial Isolates from Urine of Male and Female Students in IMO State University

The study was carried out to investigate the antibiogram of bacteria in the urine of both male and female university students. 120 mid-stream urine samples were collected from the students aged 18-25 years using sterile containers. Standard microbiological methods of sterilization and media preparation were observed. Nutrient, MacConkey and Muller Hinton media were prepared, inoculated and incubated at 370C for 24 hours. Sub-cultured colonies were identified using morphological characteristics and biochemical tests. The total heterotrophic plate bacterial counts ranged from 1.2-5.2 x 106 cfu/ml. The total coliform bacteria plate counts ranged from 1.0-4.5 x 106 cfu/ml, and the total staphylococcal plate counts ranged from 1.2-4.6 x 106 cfu/. Isolates characterized and identified from male students urine samples were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella spp, while isolates from female urine samples were Escherichia coli, Staphylococcus aureus, Proteus spp, Pseudomonas aeruginosa, and Klebsiella spp. Urine samples from female students showed more significance to urinary tract infections than those of male students. Antibiogram showed that most isolates were sensitive to Augmentin, Ceftazidime, Ciprofloxacin and Cefuroxime, while all bacterial isolates were resistant to Gentamicin and Nitrofurantoin. There is a need to examine students' urine to create awareness of urinary tract infections.