International Journal of Advanced Multidisciplinary Research and Studies
Volume 4, Issue 5, 2024
Molecular Characterization and Antimicrobial Susceptibility Profile of Bacteria found in Urine of Students in Owerri
Author(s): Ohabughiro NB, Dike KS, Maduwuba MC
Abstract:
The study was carried out to investigate the molecular characterization and antimicrobial susceptibility profile of bacteria found in urine of female university students. 200 mid-stream urine samples were collected from the female students who were aged 16-30 years, using sterile containers. Standard microbiological methods of sterilization and media preparation were observed. Nutrient, MacConkey and Muller Hinton media were prepared, inoculated and incubated at 370C for 24 hours. Bacteria were further identified using 16s rRNA gene sequencing method Subcultured colonies were identified using morphological characteristics and biochemical test. The total heterotrophic plate bacterial count ranged from 1.2-5.2 x 106 cfu/ml. The total coliform bacteria plate count ranged from 1.0-4.5 x 106 cfu/ml and the total staphylococcal plate count ranged from 1.2-4.6 x 106 cfu/. Bacterial Isolates characterized and identified from female students were Escherichia coli, Salmonella typhi, Shigella dysenteriae, Pseudomonas aeruginosa and Staphylococcus aureus. Female students showed significance to urinary tract infections. Age group of 25-30 years showed the most bacterial significant while age group 16-18 years showed the least bacterial significant. Antibiogram showed that most of the isolates were sensitive to Augmentin, Ceftazidime, Cefuroxime, Cefixime, Ciprofloxacin, Ofloxacin and while all bacterial isolates were resistant to Erythromycin, Gentamicin and Nitrofurantoin. There is need to examine the urine of female students to create awareness of the presence of urinary tract infection.
Keywords: Antibiogram, Bacteriuria, Media, Urinary Tract Infection
Pages: 662-667
Download Full Article: Click Here