Biological and Medical Efficacy of Zinc Oxide Nanoparticles Manufactured Using Saccharomyces boulardii against Burkholderia sp. Isolated from Diabetic Foot Patients

The aim of the present study is to manufacture zinc nanoparticles using the probiotic Saccharomyces boulardii and to evaluate their biological activity. The biological approach of synthesizing nanoparticles is becoming more and more important since it is more practical and ecologically friendly than chemical and physical methods of production.

Methods: Zinc acetate was added at a dose necessary to biosynthesize ZnO NPs from S. boulardii’s cellfree supernatant (1 mM).

Results: The resulting mixture's color changed from light to dark after 150 rpm of incubation, and the antibacterial activity provided confirmation that S. bularedii was in charge of this change in appearance. Atomic force microscopy (AFM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), ultraviolet-visible spectroscopy (UVS), and X-ray diffraction (XRD) all played a part in the characterization (AFM). UV-visible spectroscopy was used to measure the ZnO NPs' spectra, which were found to be (341.75 nm) in the reaction mixture. ZnO NPs had a crystal size of 29.1 nm, according to the XRD. A SEM showing the consistent, spherical shape and average size (24.61 nm) was made available. ZnO NPs in their elemental form were examined using EDS. ZnO NPs had an average diameter of (62.89 nm) and a three-dimensional structure that could be seen using AFM.The FTIR spectrum reveals a variety of functional groups that are present at various locations.Specifically, B. cepacia bacteria were identified from diabetic foot ulcers. When the produced nanoparticles were tested against the pathogenic culture of this bacteria, a very good zone of inhibition was observed.