International Journal of Advanced Multidisciplinary Research and Studies
Volume 3, Issue 3, 2023
Molecular Detection of CTX-M and Some Folate Bathway Inhibitors Resistance Genes among Gentamicin-Resistant Escherichia coli Isolates
Author(s): Nada Ayad Mohammed-Ali Doush, Nabil Salim Saaid Tuwaij
Urinary tract infections (UTIs) are one of the most common bacterial infections found in humans after respiratory tract infections because used broad-spectrum antibiotics and the indiscriminate use of antibiotics, so this study aimed to investigate genes responsible for resistance of antibiotic among gentamicin- resistant Escherichia coli isolates. Results of this study showed among 573 samples collected from urine of urinary tract infection patients, among 573 clinical specimens 102(17.80%) were male patients while 471(82.19%) were female patients pointed that 169 (35.88%) of patients had gram negative and 44 (9.34%) are gram positive. while recorded 258(82.19%) as no-growth. While males record the growth rate of gram-negative bacteria are 40(35.88%), while the gram-positive bacteria were 11 (10.78%) also recorded 51(50%) from patients as non-growth status. Results of antibiotic susceptibility showed that the highest bacterial resistance was Amikacin 29(100.0%), while Imipenem 2(6.9%) had the least resistance. Results of polymerase chain reaction (PCR) amplification showed that CTX-M gene 29/29 (100%), while folate pathway inhibitors genes among gentamicin-resistant E. coli isolates demonstrates that 82.75 percent of gentamicin-resistant E. coli isolates contained positive bands at the correct position for drf-G genes, as determined by the molecular analysis of this study. In this study, the drf-A, drf-B, and drf-K genes were not detected, according to PCR results.
Keywords: Escherichia Coli, CTX-M Gene, Folate Pathway Inhibitors Genes, Antibiotic Resistance and PCR
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